Research

Non confidential summary GLUCAN4SKIN

Non confidential summary GLUCAN4SKIN

Oxidative stress and inflammation play an important role in skin disorder and aging1. Polysaccharides, especially β-(1,3)-glucans, are abundant components of marine algae. β-(1,3)-glucans have interesting antioxidant2 and anti-inflammatory3 activities which could be advantageously optimized for potentialcosmeceutical applications. Herein we evaluated, toxicity, antioxidant and anti-inflammatory capacities of laminarin, a β-(1,3)-glucan from brown algae, on skin cells in vitro. In this project, human dermal fibroblasts adult (HDFa) and Normal human epidermal keratinocytes (NHEK) were incubated with 1, 10, 100, 500 and 1000 µg/ml of laminarin. Laminarin cytotoxicity was evaluated by a MTT assay for 24h, 48h and 72h. Oxidative stress and inflammation were induced using hydrogen peroxide (H2O2) and Lipopolysaccharide (LPS) respectively. Reactive oxygen species (ROS) production was measured using H2DCF-DA probe and IL-6 and IL-8 secretion were evaluated using ELISA assay. Our results confirmed that cells metabolic activity decreased significantly at 1000µg/ml only for HDFa. Laminarin exhibit significant antioxidant activity at 500 µg/ml only on NHEK cells. IL-8 secretion decreased significantly at 1000 µg/ml and IL-6 secretion significantly decreased at 100 and 500 µg/ml for HDFa. Our findings confirmed a positive effect of laminarin on oxidative stress and inflammation. This could be of interest in cosmetic and especially for skin aging prevention.

In terms of publication, this project is expected to lead to numerous publications in high impact newspapers. It will also serve as a springboard for applying for other funding on a national scale (mainly ANR) or even international (European project H2020).

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